Contact an Account ManagerColumn comparison of 300 Å RP phases for protein separation
Summary
Type: HPLC
Application #: 126570
Catalog#: 760186.46 - MN HPLC Column, Nucleodur C18 ec, 300A, 5um, 4.6x250mm
Detection:
Conditions: Eluent A: 0.1 % TFA in water
Eluent B: 0.08 % TFA in acetonitril
Gradient: 20 % B to 65 % B in 15 min, hold for 2 min
Flow rate: 1.3 mL/min
Temperature: 25 °C
Injection volume: 20 µL
Authors: HPLC department
Source: Macherey-Nagel, Germany 2014
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